ELUCIDATION OF A CONSERVED RNA STEM-LOOP STRUCTURE IN THE PACKAGING SIGNAL OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1

被引：41

作者：

HAYASHI, T

UENO, Y

OKAMOTO, T

机构：

[1] NATL CANC CTR,RES INST,DIV VIROL,5-1-1 TSUKIJI,CHUO KU,TOKYO 104,JAPAN

[2] SCI UNIV TOKYO,FAC PHARMACEUT SCI,SHINJUKU KU,TOKYO 162,JAPAN

来源：

FEBS LETTERS | 1993年 / 327卷 / 02期

关键词：

STEM-LOOP STRUCTURE; PACKAGING SIGNAL; HUMAN IMMUNODEFICIENCY VIRUS TYPE-1; VIRAL GENOMIC RNA; GAG PRECURSOR PROTEIN;

D O I：

10.1016/0014-5793(93)80172-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A region of human immunodeficiency virus type 1 (HIV-1) genomic RNA known to interact with the viral nucleocapsid protein is one of the crucial components for retroviral genomic RNA packaging. We have investigated the secondary structure for this RNA packaging signal of HIV-1 using RNA mapping techniques and extensive computer analyses. Our results suggest that the RNA sequence containing the packaging signal conforms to a stable stem-loop structure and that a portion of this structure is conserved among twenty independent HIV-1 isolates. The heterogeneity of the RNA packaging sequence was not correlated with the amino acid sequence variability of the viral nucleocapsid protein. These findings have critical implication in understanding the viral maturation process.

引用

页码：213 / 218

页数：6

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