A DROSOPHILA PROTEIN THAT IMPARTS DIRECTIONALITY ON A CHROMATIN INSULATOR IS AN ENHANCER OF POSITION-EFFECT VARIEGATION

被引:252
作者
GERASIMOVA, TI [1 ]
GDULA, DA [1 ]
GERASIMOV, DV [1 ]
SIMONOVA, O [1 ]
CORCES, VG [1 ]
机构
[1] RUSSIAN ACAD SCI,INST GENE BIOL,MOSCOW 117334,RUSSIA
关键词
D O I
10.1016/0092-8674(95)90031-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The suppressor of Hairy wing (su(Hw)) protein inhibits the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw)-binding sites, This polarity is due to the ability of the su(Hw)-binding region to form a chromatin insulator. Mutations in modifier of mdg4 (mod(mdg4)) enhance the effect of su(Hw) by inhibiting the function of enhancers located on both sides of the su(Hw)-binding region. This inhibition results in a variegated expression pattern, and mutations in mod(mdg4) act as classical enhancers of position-effect variegation. The mod(mdg4) and su(Hw) proteins interact with each other. The mod(mdg4) protein controls the nature of the repressive effect of su(Hw): in the absence of mod(mdg4) protein, su(Hw) exerts a bidirectional silencing effect, whereas in the presence of mod(mdg4), the silencing effect is transformed into unidirectional repression.
引用
收藏
页码:587 / 597
页数:11
相关论文
共 42 条
[1]  
Azpiazu, Frasch, tinman and bagpipe: two homeo ☐ genes that determine cell fates in dorsal mesoderm of Drosophila, Genes Dev., 7, pp. 1325-1340, (1993)
[2]  
Baxevanis, Vinson, Interactions of coiled coils in transcription factors: where is the specificity?, Curr. Opin. Genet. Dev., 3, pp. 278-285, (1993)
[3]  
Bishop, Corces, Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster, Genes & Development, 2, pp. 567-577, (1988)
[4]  
Brown, King, Wilcox, Kafatos, Developmentally regulated alternative splicing of Drosophila integrin PS2 α transcripts, Cell, 59, pp. 185-195, (1989)
[5]  
Corces, Geyer, Interactions of retrotransposons with the host genome: the case of the gypsy element of Drosophils, Trends Genet., 7, pp. 86-90, (1991)
[6]  
Chung, Whiteley, Felsenfeld, A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effects in Drosophila, Cell, 74, pp. 505-514, (1993)
[7]  
Dorn, Krauss, Reuter, Saumweber, The enhancer of position-effect variegation of Drosophila, E(var)3–93D, codes for a chromatin protein containing a conserved domain common to several transcriptional regulators, Proc. Natl. Aced. Sci. USA, 90, pp. 11376-11380, (1993)
[8]  
Georgiev, Gerasimova, Novel genes influencing the expression of the yellow locus and mdg4 (gypsy) in Drosophila melanogester, Mol. Gen. Genet., 220, pp. 121-126, (1989)
[9]  
Geyer, Corces, Separate regulatory elements are responsible for the complex pattern of tissue specific and developmental transcription of the yellow locus in Drosophile melanogaster, Genes Dev., 1, pp. 996-1004, (1987)
[10]  
Geyer, Corces, DNA position-specific repression of transcription by a Drosophila Zn finger protein, Genes Dev., 6, pp. 1865-1873, (1992)