Orientation of purple membrane in combined electric and magnetic fields

被引:14
作者
Der, A
TothBoconadi, R
Keszthelyi, L
Kramer, H
Stoeckenius, W
机构
[1] HIGH FIELD MAGNET LAB, GRENOBLE, FRANCE
[2] UNIV CALIF SANTA CRUZ, DEPT BIOL, SANTA CRUZ, CA USA
关键词
bacteriorhodopsin; orientation; electric field; magnetic field; electrooptics; photoelectric signal; magnetic susceptibility;
D O I
10.1016/0014-5793(95)01365-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The orientation of purple membrane in gels for photoelectric measurements is relatively poor, when they are prepared with the standard technique of applying a DC electric field and rapid polymerization. We have improved it by adding a high magnetic field (17.5 T) and increasing the viscosity of the membrane suspension. This process has resulted so far in a 3-fold increase of the photoelectric signals obtained. The magnetic susceptibility of purple membrane was determined.
引用
收藏
页码:419 / 420
页数:2
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