BASAL CELLS OF H-DUNNING TUMOR ARE MYOEPITHELIAL CELLS - A COMPARATIVE IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL-STUDY WITH MALE ACCESSORY SEX GLANDS AND MAMMARY-GLAND

被引:17
作者
AUMULLER, G
GROSCHELSTEWART, U
ALTMANNSBERGER, M
MANNHERZ, HG
STEINHOFF, M
机构
[1] TH DARMSTADT,DEPT ZOOL,W-6100 DARMSTADT,GERMANY
[2] UNIV GIESSEN,DEPT PATHOL,W-6300 GIESSEN,GERMANY
关键词
D O I
10.1007/BF00266961
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Recent immunohistochemical studies have shown that basal cells in human prostatic epithelium are not myoepithelial cells. Since in the literature the Dunning tumor, originally described as a rat prostate carcinoma derived from the dorsolateral prostate of a Copenhagen rat, was reported to have myoepithelial cells, a comparative immunohistochemical and ultrastructural study was performed in the H-, HIF- and AT3-lines of the Dunning tumor, the male accessory sex glands (ventral, dorsal, lateral prostate, coagulating gland, bulbourethral gland) and the mammary gland of both Copenhagen and Wistar rats. Mono- and polyclonal antibodies directed against intermediate filament proteins (cytokeratin, desmin, vimentin) and the contractile proteins (alpha-actin, muscle type specific myosin, tropomyosin) were used along with phalloidin decoration of F-actin. As in the human prostate, none of the rat prostate lobes in either strain did contain basal cells expressing cytokeratin along with alpha-actin, myosin and tropomyosin. Cells representing fully differentiated myoepithelial cells, however, were present as anticipated in the mammary gland, the bulbourethral gland and the H-tumor line of the Dunning tumor. This finding is difficult to reconcile with the contention of a prostatic origin of the H-Dunning tumor. Further studies are required to classify the epithelial parental tissue in order to define the true origin of the H-Dunning tumor and the tumor lines derived thereof.
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页码:341 / 349
页数:9
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