RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS OF CLONAL POPULATION-STRUCTURE AND GEOGRAPHIC-VARIATION IN A FRESH-WATER BRYOZOAN

被引：37

作者：

OKAMURA, B

JONES, CS

NOBLE, LR

机构：

[1] DEPT ZOOL, OXFORD OX1 3PS, ENGLAND

[2] DEPT BIOCHEM, GENET LAB, OXFORD OX1 3QU, ENGLAND

来源：

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES | 1993年 / 253卷 / 1337期

关键词：

D O I：

10.1098/rspb.1993.0095

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The randomly amplified polymorphic DNA (RAPD) assay was used to identify genetic polymorphisms in three clonal populations of the freshwater bryozoan, Cristatella mucedo, a species with few useful biochemical genetic markers. Of the 19 decamer oligonucleotide primers screened, 13 gave clear, reproducible RAPD profiles. Clonal population structure was evident, and one clone was dominant at each site. Cluster analysis grouped populations from more distant localities separately (Thames Valley and Norfolk), whereas populations from the Thames Valley clustered together. However, even at the regional scale a high degree of relatedness pertained. This work is one of the first RAPD studies of natural populations, and demonstrates the suitability of the technique for examining population structure and geographic variation in clonal taxa.

引用

页码：147 / 154

页数：8

共 36 条

[1] CHROMOSOMAL HETEROMORPHISM IN A JAPANESE POPULATION OF PECTINATELLA-GELATINOSA AND KARYOTYPIC COMPARISON WITH SOME OTHER PHYLACTOLAEMATE BRYOZOANS [J].