COMPARISON OF REGULATORY AND STRUCTURAL REGIONS OF THE XENOPUS-LAEVIS SMALL HEAT-SHOCK PROTEIN-ENCODING GENE FAMILY

被引：45

作者：

KRONE, PH ^{[1
]}

SNOW, A ^{[1
]}

ALI, A ^{[1
]}

PASTERNAK, JJ ^{[1
]}

HEIKKILA, JJ ^{[1
]}

机构：

[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ONTARIO,CANADA

来源：

GENE | 1992年 / 110卷 / 02期

关键词：

Z-DNA; ALPHA-CRYSTALLIN; DEVELOPMENTAL REGULATION; GENE DUPLICATION; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(92)90643-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated several unique Xenopus laevis hsp30 (encoding heat-shock protein 30) genomic clones, one of which contains two complete hsp30 genes (hsp30C and hsp30D), as well as the promoter and N-terminal coding region of a third gene (hsp30E). Nucleotide sequence and restriction enzyme analysis revealed that this gene cluster is different from a cluster isolated previously. The hsp30C and hsp30D genes encode proteins of approx. 24 kDa. In all, the hsp30 gene family contains a minimum of seven genes. The strand exchange and breakage of the duplication events which generated this gene family appear to have occurred within tracts of DNA which potentially can assume a Z-DNA conformation. Comparing the amino acid (aa) sequences of each known Hsp30 protein with bovine alpha-crystallin revealed a high degree of shared conservation of aa that constitute the major structural feature(s) of alpha-crystallin.

引用

页码：159 / 166

页数：8

共 35 条

[1] SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].

BENTON, WD ;

DAVIS, RW .

SCIENCE, 1977, 196 (4286) :180-182

[2] HEAT-SHOCK REGULATORY ELEMENTS FUNCTION AS AN INDUCIBLE ENHANCER IN THE XENOPUS HSP70 GENE AND WHEN LINKED TO A HETEROLOGOUS PROMOTER [J].

BIENZ, M ;

PELHAM, HRB .

CELL, 1986, 45 (05) :753-760

[3] XENOPUS-HSP 70 GENES ARE CONSTITUTIVELY EXPRESSED IN INJECTED OOCYTES [J].

BIENZ, M .

EMBO JOURNAL, 1984, 3 (11) :2477-2483

[4] DEVELOPMENTAL CONTROL OF THE HEAT-SHOCK RESPONSE IN XENOPUS [J].

BIENZ, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (10) :3138-3142

[5]

BONNIEU A, 1990, ONCOGENE, V5, P1585

[6] DETERMINANTS OF MESSENGER-RNA STABILITY [J].

BRAWERMAN, G .

CELL, 1987, 48 (01) :5-6

[7]

Browder L W, 1989, Dev Biol (N Y 1985), V6, P97

[8] STRUCTURE, ORGANIZATION, AND EXPRESSION OF THE 16-KDA HEAT-SHOCK GENE FAMILY OF CAENORHABDITIS-ELEGANS [J].

CANDIDO, EPM ;

JONES, D ;

DIXON, DK ;

GRAHAM, RW ;

RUSSNAK, RH ;

KAY, RJ .

GENOME, 1989, 31 (02) :690-697

[9] SIMPLE DNA-SEQUENCES IN HOMOLOGOUS FLANKING REGIONS NEAR IMMUNOGLOBULIN VH GENES - A ROLE IN GENE INTERACTION [J].

COHEN, JB ;

EFFRON, K ;

RECHAVI, G ;

BEN-NERIAH, Y ;

ZAKUT, R ;

GIVOL, D .

NUCLEIC ACIDS RESEARCH, 1982, 10 (11) :3353-3370

[10] HEAT-SHOCK GENE-EXPRESSION IN XENOPUS-LAEVIS A6 CELLS IN RESPONSE TO HEAT-SHOCK AND SODIUM ARSENITE TREATMENTS [J].

DARASCH, S ;

MOSSER, DD ;

BOLS, NC ;

HEIKKILA, JJ .

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1988, 66 (08) :862-870

← 1 2 3 4 →