OPTIMIZATION OF SUPEROVULATION IN THE REPRODUCTIVELY MATURE MOUSE

被引：21

作者：

LEGGE, M

SELLENS, MH

机构：

[1] Department of Biology, University of Essex, Colchester, CO4 3SQ, Wivenhoe Park

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1994年 / 11卷 / 06期

关键词：

SUPEROVULATION; MOUSE OPTIMIZATION OOCYTES; BLASTOCYSTS; OVARIES;

D O I：

10.1007/BF02215719

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Optimum gonadotropin doses and chronology were established for the induction of superovulation in sexually mature hybrid mice (BALB/cBy x C57BL/6By). A regime of 12 IU pregnant mares' serum gonadotropin (PMSG), followed 48 hr later by 20 IU human chorionic gonadotropin (hCG) administered 1 hr before the midpoint of the light cycle (1200), gave the maximum ovulatory response. There was no evidence that endogenous luteinizing hormone influenced the superovulation response to exogenous gonadotropins. Fewer than 50% of zygotes reached the blastocyst stage (90-93 hr post hCG), with the grentest rate of loss at the two- to four-cell stage. Litter size following superovulation was 19.6 +/- 0.9. There was no significant difference between the number of blastocysts observed and litter size. Similarly, counts of mature follicles in ovaries prior to hCG stimulation were not significantly greater than the number of secondary oocytes that subsequently ovulated. These data indicate that standard superovulation protocols may require fine-tuning to maximize productivity and confirm that embryo loss is greatest between the first cleavage division and blastocyst formation.

引用

页码：312 / 318

页数：7

共 34 条

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