ACTIVATION OF EXOCYTOSIS BY THE HETEROTRIMERIC-G PROTEIN-G(I3)

被引：230

作者：

ARIDOR, M

RAJMILEVICH, G

BEAVEN, MA

SAGIEISENBERG, R

机构：

[1] NHLBI,CHEM PHARMACOL LAB,BLDG 10,ROOM 8H108,BETHESDA,MD 20892

[2] WEIZMANN INST SCI,DEPT CHEM IMMUNOL,IL-76100 REHOVOT,ISRAEL

来源：

SCIENCE | 1993年 / 262卷 / 5139期

关键词：

D O I：

10.1126/science.7504324

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Secretagogues of rat peritoneal mast cells, such as mastoparan and compound 48/80, induce mast cell exocytosis by activating directly the guanosine triphosphate-binding proteins that are required for exocytosis. The introduction of a synthetic peptide that corresponds to the carboxyl-terminal end sequence of Galpha(i3) into the cells specifically blocked this secretion. Similar results were obtained when antibodies to this peptide were introduced. The Galpha(i3) was located in both the Golgi and the plasma membrane, but only the latter source of Galpha(i3) appeared to be essential for secretion. These results indicate that Galpha(i3) functions to control regulated exocytosis in mast cells.

引用

页码：1569 / 1572

页数：4

共 27 条

[1] NEOMYCIN IS A POTENT SECRETAGOGUE OF MAST-CELLS THAT DIRECTLY ACTIVATES A GTP-BINDING PROTEIN INVOLVED IN EXOCYTOSIS [J].