FLUOROMETRIC-DETERMINATION OF ISONIAZID AND ITS METABOLITES IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

KUBO, H

KINOSHITA, T

MATSUMOTO, K

NISHIKAWA, T

机构：

[1] TOHO UNIV,SCH PHARMACEUT SCI,FUNABASHI,CHIBA 274,JAPAN

[2] KITASATO UNIV,SCH MED,SAGAMIHARA,KANAGAWA 228,JAPAN

来源：

CHROMATOGRAPHIA | 1990年 / 30卷 / 1-2期

关键词：

Column liquid chromatography; Fluorometric detection; Isoniazid metabolites in urine; Post column derivatization;

D O I：

10.1007/BF02270451

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, simple, and accurate method was developed for the determination of isoniazid and its metabolites (isonicotinic acid, acetylisoniazid and isonicotinylglycine) in urine by high-performance liquid chromatography. Urine is diluted with the mobile phase. After centrifugation, an aliquot of the supernatant is injected into the chromatograph. Isoniazid and its metabolites are separated by reversed-phase ionpairing chromatography with a mobile phase containing propanesulfonate and detected by fluorometry using postcolumn derivatization at high temperature (150°C) with hydrogen peroxide. The method was applied to the analysis of urine from patients receiving isoniazid therapy. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：69 / 72

页数：4

共 8 条

[1] PHARMACOKINETICS OF ISONIAZID METABOLISM IN MAN [J].