EXPERIMENTAL HYBRID ISLET TRANSPLANTATION - APPLICATION OF POLYVINYL-ALCOHOL MEMBRANE FOR ENTRAPMENT OF ISLETS

被引：56

作者：

INOUE, K

FUJISATO, T

GU, YJ

BURCZAK, K

SUMI, S

KOGIRE, M

TOBE, T

UCHIDA, K

NAKAI, I

MAETANI, S

IKADA, Y

机构：

[1] First Department of Surgery, Kyoto University, Kyoto

[2] Research Center for Biomedical Engineering, Kyoto University, Kyoto

[3] Institute of Applied Radiation Chemistry, Technical University of Lodz, Lodz

[4] College of Medical Technology, Kyoto University

[5] Second Department of Surgery, Kyoto Prefectural Medical School, Kyoto

来源：

PANCREAS | 1992年 / 7卷 / 05期

关键词：

BIOARTIFICIAL PANCREAS; DIABETIC RATS; HYBRID ISLET; IMMUNOISOLATION; MESH-REINFORCED POLYVINYL ALCOHOL TUBE (MRPT); POLYVINYL ALCOHOL MEMBRANE; TRANSPLANTATION;

D O I：

10.1097/00006676-199209000-00009

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

In this study, we first examined in vitro a polyvinyl alcohol membrane to be used to contain hybrid islet cells, and second we tested a bioartificial pancreas with entrapment of pancreatic islets in polyvinyl alcohol membrane in rats with experimentally induced diabetes. The permeability of the polyvinyl alcohol membrane to different substances was studied in a two-cell chamber system. Glucose, insulin, and nutrients passed through the membrane easily, whereas the passage of immunoglobulin G was completely prevented, indicating that this membrane could be effective in protecting the bioartificial pancreas from immunorejection. Approximately 2,000 islets collected from three Sprague-Dawley rats were enclosed in a mesh-reinforced polyvinyl alcohol tube and transplanted into the peritoneal cavity of six Wistar rats with streptozotocin-induced diabetes. Their nonfasting serum glucose levels were significantly decreased for at least 12 days. Six diabetic rats receiving intraperitoneal transplantation of free islets without the tube showed a slight but significant decrease in nonfasting serum glucose levels for only 3 days. One diabetic rat with transplantation of the bioartificial pancreas had a significant and sustained decrease in nonfasting glucose levels from pretransplanted levels of 440-500 mg/dt to a mean value of 162 +/- 13 mg/dl for over 3 months without immunosuppression. The bioartificial pancreas was then removed, and glucose levels gradually increased to over 500 mg/dl. The results of the present study suggest that a bioartificial pancreas with entrapment of islets in a polyvinyl alcohol membrane could be a promising therapeutic approach to diabetes mellitus.

引用

页码：562 / 568

页数：7

共 17 条

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