INFLUENCE OF AGITATION RATE ON GROWTH AND RIBONUCLEASE PRODUCTION BY FREE AND IMMOBILIZED ASPERGILLUS-CLAVATUS CELLS

被引：17

作者：

MANOLOV, RJ

机构：

[1] Institute of Microbiology, Department of Enzymes, Bulgarian Academy of Sciences, Sofia, 1113

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 33卷 / 03期

关键词：

IMMOBILIZED CELLS; ASPERGILLUS-CLAVATUS; RIBONUCLEASE; POLY(VINYL ALCOHOL); CRYOSTRUCTURIZATION; AGITATION; BATCH BIOREACTOR;

D O I：

10.1007/BF02921831

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aspergillus clavatus spores have been immobilized in poly(vinyl alcohol) criogel (PVAC) for ribonucleae (RNase) production. The enzyme productivity and growth of free (FC) and immobilized cells (IC) were studied in a bioreactor with agitation under different cultivation conditions. The influence of some medium compunds, aeration, and agitation speeds were investigated. Production of RNase was stimulated by the presence of reducing glucose, peptone, and soybean concentrations in the medium. From 42,000 to 45,000 U/L were produced by IC using optimized batch fermentation conditions. The RNase prouction by IC was 2.3 and 2.5 times greater than the same by FC in a bioreactor and control flasks, respectively.

引用

页码：157 / 167

页数：11

共 15 条

[1]

BAILEY MJ, 1989, APPL MICROBIOL BIOT, V30, P5, DOI 10.1007/BF00255989

[2]

BEZBORODOVA S. I., 1968, MIKROBIOLOGIYA, V37, P10

[3] THE COMPLETE AMINO-ACID-SEQUENCE OF RIBONUCLEASE-C-2 FROM ASPERGILLUS-CLAVATUS [J].