CHARACTERIZATION AND LOCALIZATION OF THE FMR-1 GENE-PRODUCT ASSOCIATED WITH FRAGILE-X SYNDROME

被引:288
作者
VERHEIJ, C [1 ]
BAKKER, CE [1 ]
DEGRAAFF, E [1 ]
KEULEMANS, J [1 ]
WILLEMSEN, R [1 ]
VERKERK, AJMH [1 ]
GALJAARD, H [1 ]
REUSER, AJJ [1 ]
HOOGEVEEN, AT [1 ]
OOSTRA, BA [1 ]
机构
[1] ERASMUS UNIV ROTTERDAM,MGC DEPT CLIN GENET,POB 1738,3000 DR ROTTERDAM,NETHERLANDS
关键词
D O I
10.1038/363722a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
THE fragile X syndrome is the most frequent form of inherited mental retardation after Down's syndrome, having an incidence of one in 1,250 males1,2. The fragile X syndrome results from amplification of the CGG repeat found in the FMR-1 gene3-6. This CGG repeat shows length variation in normal individuals and is increased significantly in both carriers and patients3-6; it is located 250 base pairs distal to a CpG island6 which is hypermethylated in fragile X patients4-7. The methylation probably results in downregulation of FMR-1 gene expression8. No information can be deduced about the function of the FMR-1 protein from its predicted sequence. Here we investigate the nature and function of the protein encoded by the FMR-1 gene using polyclonal antibodies raised against the predicted amino-acid sequences. Four different protein products, possibly resulting from alternative splicing, have been identified by immunoblotting in lymphoblastoid cell lines of healthy individuals. All these proteins were missing in cell lines from patients not expressing FMR-1 messenger RNA. The intracellular localization of the FMR-1 gene products was investigated by transient expression in COS-1 cells and found to be cytoplasmic. Localization was also predominantly cytoplasmic in the epithelium of the oesophagus, but in some cells was obviously nuclear.
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页码:722 / 724
页数:3
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