PLATELET-DERIVED GROWTH FACTOR-B CHAIN HOMODIMER ENHANCES CHEMOTAXIS AND DNA-SYNTHESIS IN NORMAL OSTEOBLAST-LIKE CELLS (MC3T3-E1)

被引：51

作者：

TSUKAMOTO, T

MATSUI, T

FUKASE, M

FUJITA, T

机构：

[1] Third Division, Department of Medicine, Kobe University School of Medicine, Kobe

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91629-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate that recombinant platelet-derived growth factor B chain homodimer (PDGF-BB) could induce both the chemotactic activity and the DNA synthesis in a normal osteoblast-like cell line (MC3T3-E1). Cell migration toward recombinant human PDGF-BB was observed with the modified Boyden chamber technique. Maximum chemotaxis was exhibited at 25 ng/ml of PDGF-BB. DNA synthesis as indicated by [3H]-thymidine incorporation was also enhanced about 4-fold at 25-100 ng/ml PDGF-BB. Our results suggested that PDGF might be one of the candidates among local coupling factors for bone remodeling. © 1991.

引用

页码：745 / 751

页数：7

共 17 条

[1] COMPARISON OF BIOLOGICAL PROPERTIES AND TRANSFORMING POTENTIAL OF HUMAN PDGF-A AND PDGF-B CHAINS [J].