ALVEOLAR EPITHELIAL INJURY AND PLEURAL EMPYEMA IN ACUTE PSEUDOMONAS-AERUGINOSA PNEUMONIA IN ANESTHETIZED RABBITS

被引:102
作者
WIENERKRONISH, JP [1 ]
SAKUMA, T [1 ]
KUDOH, I [1 ]
PITTET, JF [1 ]
FRANK, D [1 ]
DOBBS, L [1 ]
VASIL, ML [1 ]
MATTHAY, MA [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST,DEPT MED,SAN FRANCISCO,CA 94143
关键词
PLEURAL SPACE; LUNG INJURY; PSEUDOMONAS-AERUGINOSA ISOGENIC STRAINS; PHOSPHOLIPASE-C; ELASTASE; EXOENZYME-S; ALVEOLAR EPITHELIUM;
D O I
10.1152/jappl.1993.75.4.1661
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We developed an experimental model of acute Pseudomonas aeruginosa pneumonia in anesthetized ventilated rabbits to determine whether bacterial-induced injury to the alveolar epithelium would occur and the effect of the injury on the pleural space. Dose-response studies established that 10(9) colony-forming units of P. aeruginosa (wild-type strain, PAO-1) were required to injure the epithelial barrier and to cause pleural empyema with exudative pleural effusions that contained both the instilled alveolar protein tracer and P. aeruginosa. We explored the mechanisms of P. aeruginosa-induced lung and pleural injury by using three isogenic bacterial strains to compare several extracellular virulence products. PAO-S21, which carries an insertion mutation in a regulatory gene that prevents the production of exoenzyme S, resulted in no lung or pleural injury. PAO-R1, which carries a deletion in a regulatory gene that controls the production of elastase and alkaline protease, caused the same degree of lung and pleural injury as PAO-1 did. Instillation of PLC-SRN, which has both structural genes encoding phospholipase C activity deleted, resulted in a moderate reduction in alveolar epithelial injury. Although other products may be involved, exoenzyme S and phospholipase C are important in mediating injury to the alveolar epithelial barrier in acute P. aeruginosa pneumonia in rabbits.
引用
收藏
页码:1661 / 1669
页数:9
相关论文
共 43 条
  • [1] EFFECTS OF HUMAN NEUTROPHIL ELASTASE AND PSEUDOMONAS-AERUGINOSA PROTEINASES ON HUMAN RESPIRATORY EPITHELIUM
    AMITANI, R
    WILSON, R
    RUTMAN, A
    READ, R
    WARD, C
    BURNETT, D
    STOCKLEY, RA
    COLE, PJ
    [J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1991, 4 (01) : 26 - 32
  • [2] AZGHANI A O, 1991, Journal of Cell Biology, V115, p481A
  • [3] EFFECTS OF PSEUDOMONAS-AERUGINOSA ELASTASE ON ALVEOLAR EPITHELIAL PERMEABILITY IN GUINEA-PIGS
    AZGHANI, AO
    CONNELLY, JC
    PETERSON, BT
    GRAY, LD
    COLLINS, ML
    JOHNSON, AR
    [J]. INFECTION AND IMMUNITY, 1990, 58 (02) : 433 - 438
  • [4] ALTERATIONS OF GAS-EXCHANGE APPARATUS IN ADULT RESPIRATORY INSUFFICIENCY ASSOCIATED WITH SEPTICEMIA
    BACHOFEN, M
    WEIBEL, ER
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1977, 116 (04): : 589 - 615
  • [5] PSEUDOMONAS-AERUGINOSA EXOENZYME-S IS AN ADHESIN
    BAKER, NR
    MINOR, V
    DEAL, C
    SHAHRABADI, MS
    SIMPSON, DA
    WOODS, DE
    [J]. INFECTION AND IMMUNITY, 1991, 59 (09) : 2859 - 2863
  • [6] ALVEOLAR LIQUID AND PROTEIN CLEARANCE FROM NORMAL DOG LUNGS
    BERTHIAUME, Y
    BROADDUS, VC
    GROPPER, MA
    TANITA, T
    MATTHAY, MA
    [J]. JOURNAL OF APPLIED PHYSIOLOGY, 1988, 65 (02) : 585 - 593
  • [7] BETA-ADRENERGIC AGONISTS INCREASE LUNG LIQUID CLEARANCE IN ANESTHETIZED SHEEP
    BERTHIAUME, Y
    STAUB, NC
    MATTHAY, MA
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1987, 79 (02) : 335 - 343
  • [8] CLEARANCE OF LUNG EDEMA INTO THE PLEURAL SPACE OF VOLUME-LOADED ANESTHETIZED SHEEP
    BROADDUS, VC
    WIENERKRONISH, JP
    STAUB, NC
    [J]. JOURNAL OF APPLIED PHYSIOLOGY, 1990, 68 (06) : 2623 - 2630
  • [9] BACTEREMIC NOSOCOMIAL PNEUMONIA - ANALYSIS OF 172 EPISODES FROM A SINGLE METROPOLITAN AREA
    BRYAN, CS
    REYNOLDS, KL
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1984, 129 (05): : 668 - 671
  • [10] COBURN J, 1992, CURR TOP MICROBIOL, V175, P133