METHODS AND REAGENTS - CLONING PCR PRODUCTS USING T-VECTORS

被引：9

作者：

HENGEN, PN

机构：

[1] National Cancer Institute, Frederick Cancer Research and Development Center, Frederick

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1995年 / 20卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0968-0004(00)88964-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column compares some of the commercially available vectors for cloning PCR fragments and discusses some of the problems encountered with them. For details on how to partake in the newsgroup, see the accompanying box.

引用

页码：85 / 86

页数：2

共 4 条

[1] NOVEL NON-TEMPLATED NUCLEOTIDE ADDITION-REACTIONS CATALYZED BY PROCARYOTIC AND EUKARYOTIC DNA-POLYMERASES
CLARK, JM
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (20) : 9677 - 9686
[2] GENERAL-METHOD FOR DIRECT CLONING OF DNA FRAGMENTS GENERATED BY THE POLYMERASE CHAIN-REACTION
KOVALIC, D
KWAK, JH
WEISBLUM, B
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (16) : 4560 - 4560
[3] CONSTRUCTION OF T-VECTORS, A RAPID AND GENERAL SYSTEM FOR DIRECT CLONING OF UNMODIFIED PCR PRODUCTS
MARCHUK, D
DRUMM, M
SAULINO, A
COLLINS, FS
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (05) : 1154 - 1154
[4] A UNIVERSAL METHOD FOR THE DIRECT CLONING OF PCR AMPLIFIED NUCLEIC-ACID
MEAD, DA
PEY, NK
HERRNSTADT, C
MARCIL, RA
SMITH, LM
[J]. BIO-TECHNOLOGY, 1991, 9 (07): : 657 - 663

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