DETECTION AND GENOTYPING OF HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 BY POLYMERASE CHAIN-REACTION

被引：24

作者：

LUCOTTE, G ^{[1
]}

BATHELIER, C ^{[1
]}

LESPIAUX, V ^{[1
]}

BALI, C ^{[1
]}

CHAMPENOIS, T ^{[1
]}

机构：

[1] CLAUDE LEVY LAB,PARIS 14,FRANCE

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 05期

关键词：

HERPES SIMPLEX VIRUS; POLYMERASE CHAIN REACTION; TYPE-SPECIFIC OLIGONUCLEOTIDE PRIMERS;

D O I：

10.1016/S0890-8508(95)91508-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and typing of herpes simplex virus (HSV) types 1 and 2. It was possible to detect and type HSV using two primers pairs in a simultaneous double PCR reaction, where the type of HSV present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. This PCR assay was tested on about 500 clinical specimens. (C) 1995 Academic Press Limited

引用

页码：287 / 290

页数：4

共 15 条

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