Amperometric determination of cholesterol in serum with use of a renewable surface peroxidase electrode

被引：73

作者：

Charpentier, L ^{[1
]}

ElMurr, N ^{[1
]}

机构：

[1] FAC SCI & TECH, GRP ELECTOCHIM, LAB RMN & REACT CHIM, URA 742, F-44072 NANTES 03, FRANCE

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 318卷 / 01期

关键词：

biosensors; amperometry; enzymatic methods; cholesterol; peroxidase; cholesterol oxidase; cholesterol ester hydrolase;

D O I：

10.1016/0003-2670(95)00311-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive modified carbon paste electrode for the direct determination elf cholesterol is described. Both cyclic voltammetric and chronoamperometric techniques are used. The electrode was modified with hydroxymethyl ferrocene and horseradish peroxidase. Cholesterol oxidase and cholesterol ester hydrolase are used in solution. The measured current is linear with cholesterol concentration in the range 1 X 10(-6) to 1.5 X 10(-4) M. The measuring time is 30 s.

引用

页码：89 / 93

页数：5

共 31 条

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