SPECIFIC LIQUID DNA HYBRIDIZATION KINETICS MEASURED BY FLUORESCENCE POLARIZATION

被引：13

作者：

HERNING, T

TAMIYA, E

KARUBE, I

KOBAYASHI, S

机构：

[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,4-6-1 KOMABA,MEGURO KU,TOKYO 153,JAPAN

[2] KAO CORP,KAO INST FUNDAMENTAL RES,2606 OAZA,ICHIKAI,TOCHIGI,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 244卷 / 02期

关键词：

FLUOROMETRY; KINETIC ANALYSIS; DNA HYBRIDIZATION KINETICS; FLUORESCENCE POLARIZATION;

D O I：

10.1016/S0003-2670(00)82500-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The kinetics of specific DNA hybrid formation were monitored directly in solution. Detection was based on fluorescence polarization measurements of labelled oligonucleotides at different stages during the hybridization. The effect of mismatched base pairs on the kinetics was measured. Significant differences could be observed in the kinetics when as few as three mismatches were introduced by the polymerase chain reaction technique in the target DNA sequences.

引用

页码：207 / 213

页数：7

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