MOLECULAR ANALYSIS OF 2 YPT/RAB-RELATED SEQUENCES ISOLATED FROM SOYBEAN (GLYCINE-MAX) DNA LIBRARIES

被引:12
作者
BORG, S [1 ]
POULSEN, C [1 ]
机构
[1] AARHUS UNIV,DEPT MOLEC BIOL,GENE EXPRESS LAB,DK-8000 AARHUS,DENMARK
关键词
GENOMIC SEQUENCE; FILTER GTP-BINDING ASSAYS; LEGUMES; POLYMERASE CHAIN REACTION; SMALL GTP-BINDING PROTEINS; TRANSCRIPT ANALYSIS;
D O I
10.1007/BF00039530
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
From nodule and seedling cDNA libraries we isolated cDNA copies of two mRNAs, derived from the genes gmr1 and gmr2, encoding members of the Ypt/Rab family of small GTP-binding proteins. Two deduced protein products, GMR1 and GMR2, were found to be nearly identical differing by only four amino acids in the analysed parts. The two putative proteins are 79% identical to the previously described ARA small GTPase from Arabidopsis thaliana. The GMR proteins may thus be the counterpart of the ARA protein and may perform a related biological function in Glycine Max. The gmr2 genomic sequence was isolated and structurally analysed. Expression analyses by northern and cDNA-based PCR showed that the gmr1 and gmr2 genes are constitutively expressed in different plant organs, although at a slightly higher level in callus culture. The classification of the gmr sequences as relatives of the Ypt/Rab family suggests that the deduced GMR proteins are involved in control of processes related to vesicle trafficking in plant cells.
引用
收藏
页码:175 / 187
页数:13
相关论文
共 43 条
[1]  
ANAI T, 1991, GENE, V108, P259, DOI 10.1016/0378-1119(91)90442-E
[2]   CDNA CLONING OF COMPONENT-A OF RAB GERANYLGERANYL TRANSFERASE AND DEMONSTRATION OF ITS ROLE AS A RAB ESCORT PROTEIN [J].
ANDRES, DA ;
SEABRA, MC ;
BROWN, MS ;
ARMSTRONG, SA ;
SMELAND, TE ;
CREMERS, FPM ;
GOLDSTEIN, JL .
CELL, 1993, 73 (06) :1091-1099
[3]   MOLECULAR CHARACTERIZATION OF AN ARABIDOPSIS-THALIANA CDNA-ENCODING A SMALL GTP-BINDING PROTEIN, RHA1 [J].
ANUNTALABHOCHAI, S ;
TERRYN, N ;
VANMONTAGU, M ;
INZE, D .
PLANT JOURNAL, 1991, 1 (02) :167-174
[4]  
AUSUBEL FM, 1990, CURRENT PROTOCOLS MO
[5]  
BALCH WE, 1989, J BIOL CHEM, V264, P16965
[6]  
BALCH WE, 1988, TRENDS BIOCHEM SCI, V53, P669
[7]  
BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0
[8]   DO GTPASES DIRECT MEMBRANE TRAFFIC IN SECRETION [J].
BOURNE, HR .
CELL, 1988, 53 (05) :669-671
[9]   THE GTPASE SUPERFAMILY - A CONSERVED SWITCH FOR DIVERSE CELL FUNCTIONS [J].
BOURNE, HR ;
SANDERS, DA ;
MCCORMICK, F .
NATURE, 1990, 348 (6297) :125-132
[10]   RAIN-INDUCED, WIND-INDUCED, AND TOUCH-INDUCED EXPRESSION OF CALMODULIN AND CALMODULIN-RELATED GENES IN ARABIDOPSIS [J].
BRAAM, J ;
DAVIS, RW .
CELL, 1990, 60 (03) :357-364