HISTOCHEMICAL STUDY OF ENZYME SYSTEMS IN FROZEN DRIED TISSUE

Cytochrome oxidase, succinic dehydrogenase, phosphorylase and branching enzymes were consistently demonstrated in frozen dried paraffin embedded dog myocardium if the appropriate solvent for deparaffinization was used. No modification of the incubating medium was necessary for cytochrome oxidase. For succinic dehydrogenase a 0.8 M succinate solution was used instead of the usual 0.2 M solution and phenazine methosulfate had to be added to the incubating medium. No primer or activators were necessary for demonstration of phosphorylase and branching enzyme activities. © 1968 Springer-Verlag.