AUTOMATED SEPARATION AND QUANTITATION OF LIPID FRACTIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND MASS DETECTION

被引：32

作者：

REDDEN, PR ^{[1
]}

HUANG, YS ^{[1
]}

机构：

[1] EFAMOL RES INST,POB 818,KENTVILLE B4N 4H8,NS,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 567卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80305-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This report describes an improved separation and quantitation of lipid fractions in a total lipid extract by high-performance liquid chromatography using a modified solvent and gradient system delivered by dual pumps and incorporating a mass detector and autosampler. The detector responses for various lipid fractions (cholesteryl esters, triacylglycerols, free cholesterol, and seven major phospholipid classes) were fitted to a quadratic equation, y = ax2 + bx + c, and quantified after detector calibration by a computer. This new system has the advantage of automation and reproducible separation. The present method was applied to rat liver analysis.

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页码：21 / 27

页数：7

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