PROTEIN-PRODUCTION AND THE MOLTING CYCLE IN THE CRAYFISH ASTACUS-LEPTODACTYLUS (NORDMANN, 1842) .2. HEMOCYANIN AND PROTEIN-SYNTHESIS IN THE MIDGUT GLAND

The midgut gland from the crayfish Astacus leptodactylus synthesizes and secretes hemocyanin and proteins linearly for at least 24 hr in vitro. There are no sex-specific differences in the rate of protein and hemocyanin synthesis. During a standard incubation of 5 hr, up to 64% of the newly synthesized protein represents hemocyanin, which is also the predominant secretory protein. There are pronounced changes in the rate of hemocyanin synthesis during the molting cycle which coincide with corresponding changes in total protein synthesis. The titer of protein and hemocyanin synthesis during an intermolt phase exhibits a biphasic profile with the highest values in intermolt stage C4 and in premolt stage D1. © 1992.