EFFECT OF HYDRATION ON THE MORPHOLOGY OF ENZYME POWDER

被引:24
作者
ROZIEWSKI, K
RUSSELL, AJ
机构
[1] UNIV PITTSBURGH,CTR BIOTECHNOL & BIOENGN,PITTSBURGH,PA 15261
[2] UNIV PITTSBURGH,DEPT CHEM ENGN,PITTSBURGH,PA 15261
关键词
ENZYMES; ORGANIC SOLVENTS; HYDRATION; ENVIRONMENTAL ELECTRON MICROSCOPY;
D O I
10.1002/bit.260391114
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We report the first direct images of the hydration of protein powders. Using an environmental scanning electron microscope (ESEM) we have taken a series of micrographs of a region of the enzyme (subtilisin) powder whilst hydrating the sample. In addition, the sample has been viewed during exposure to toluene vapors. The ESEM is a remarkable new instrument that will have wide applicability in imaging of biological materials in their native environments.
引用
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页码:1171 / 1175
页数:5
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