PURIFICATION AND PROPERTIES OF ALPHA-GLUCOSIDASE FROM TRICHODERMA-VIRIDE

被引：4

作者：

YAMASAKI, Y ^{[1
]}

ELBEIN, AD ^{[1
]}

KONNO, H ^{[1
]}

机构：

[1] UNIV ARKANSAS MED SCI HOSP, DEPT BIOCHEM & MOLEC BIOL, LITTLE ROCK, AR 72205 USA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 11期

关键词：

D O I：

10.1271/bbb.59.2181

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Glucosidase (EC 3.2.1.20) from Trichoderma viride was purified by a procedure including Sephacryl S-200 HR column chromatography, preparative isoelectric focusing, and Snperdex 200 HR column chromatography. The enzyme was found to have an apparent molecular weight of 67,000 on SDS/PAGE and 65,000 on gelfiltration, showing that the enzyme is a single peptide, The isoelectric point of the enzyme was 5.0. The enzyme hydrolyzed alpha-1,4-glucosidic linkage more rapidly than alpha-1,6-glucosidic and alpha-1,3-glucosidic linkages, The enzyme was weakly inhibited by castanospermine.

引用

页码：2181 / 2182

页数：2

共 18 条

[1] SUBSTRATE-SPECIFICITY OF FLINT CORN ALPHA-GLUCOSIDASE [J].

CHIBA, S ;

SHIMOMURA, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1975, 39 (05) :1041-1047

[2] STUDIES ON ANOMERIC FORMS OF PRODUCTS OF CARBOHYDRASES .1. QUANTITATIVE STUDY OF ANOMERIC FORMS OF GLUCOSE PRODUCED BY ALPHA-GLUCOSIDASES AND GLUCOAMYLASES [J].

CHIBA, S ;

KIMURA, A ;

MATSUI, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1983, 47 (08) :1741-1746

[3] DETERMINATION OF MALTASE AND ISOMALTASE ACTIVITIES WITH A GLUCOSE-OXIDASE REAGENT [J].

DAHLQVIST, A .

BIOCHEMICAL JOURNAL, 1961, 80 (03) :547-&

[4]

GRINNA LS, 1979, J BIOL CHEM, V254, P8814

[5] STUDIES ON CARBOHYDRATE-METABOLIZING ENZYMES - HYDROLYSIS OF ALPHA-GLUCOSIDES INCLUDING NIGEROSE BY EXTRACTS OF ALFALFA AND OTHER HIGHER PLANTS [J].

HUTSON, DH ;

MANNERS, DJ .

BIOCHEMICAL JOURNAL, 1965, 94 (03) :783-&

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7] ENZYMIC MECHANISM OF STARCH BREAKDOWN IN GERMINATING RICE SEEDS .2. SCUTELLUM AS SITE OF SUCROSE SYNTHESIS [J].

NOMURA, T ;

KONO, Y ;

AKAZAWA, T .

PLANT PHYSIOLOGY, 1969, 44 (05) :765-+

[8] PURIFICATION AND SUBSTRATE-SPECIFICITY OF ALPHA-GLUCOSIDASE FROM PAECILOMYCES-VARIOTI AHU 9417 [J].

OGUMA, T ;

MATSUI, H ;

TANIDA, M ;

TAKAO, S ;

HONMA, M ;

CHIBA, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (12) :1906-1910

[9] CASTANOSPERMINE INHIBITS THE PROCESSING OF THE OLIGOSACCHARIDE PORTION OF THE INFLUENZA VIRAL HEMAGGLUTININ [J].

PAN, YT ;

HORI, H ;

SAUL, R ;

SANFORD, BA ;

MOLYNEUX, RJ ;

ELBEIN, AD .

BIOCHEMISTRY, 1983, 22 (16) :3975-3984

[10] DETERMINATION OF NEURAMINIC (SIALIC) ACID, GLUCOSE AND FRUCTOSE IN SPINAL FLUID [J].

PAPADOPOULOS, NM ;

HESS, WC .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1960, 88 (01) :167-171

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