SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17

被引：21

作者：

CUNNINGHAM, C

MCPHERSON, CA

MARTIN, J

HARRIS, WJ

FLINT, HJ

机构：

[1] UNIV ABERDEEN MARISCHAL COLL,DEPT MOLEC & CELLULAR BIOL,ABERDEEN AB9 1AS,SCOTLAND

[2] ROWETT RES INST,BUCKSBURN AB2 9SB,ABERDEEN,SCOTLAND

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 228卷 / 1-2期

关键词：

CELLULASE GENE SEQUENCE; RUMINOCOCCUS-FLAVEFACIENS; RUMEN BACTERIA; ENDOGLUCANASE GENE; CELLULASE FAMILIES;

D O I：

10.1007/BF00282484

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

引用

页码：320 / 323

页数：4

共 18 条

[1] MOLECULAR-BIOLOGY OF CELLULOSE DEGRADATION [J].