ACCURATE TRANSCRIPTION INITIATION OF VICIA-FABA RDNA IN A WHOLE CELL EXTRACT FROM EMBRYONIC AXES

被引：10

作者：

YAMASHITA, J ^{[1
]}

NAKAJIMA, T ^{[1
]}

TANIFUJI, S ^{[1
]}

KATO, A ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC SCI,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN

来源：

PLANT JOURNAL | 1993年 / 3卷 / 01期

关键词：

D O I：

10.1111/j.1365-313X.1993.tb00022.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An in vitro assay system is presented for analyses of transcription of the Vicia faba rRNA gene. The system consists of a whole cell extract from the embryonic axes of V. faba, substrates and exogenously added rDNA fragments. Specific initiation at the promoter was determined by a size analysis of the transcripts synthesized from DNA templates of various molecular sizes. This transcription was dependent on RNA polymerase I, since the reaction mixtures contain alpha-amanitin. Furthermore, the primer extension experiment showed that at least a part of each transcript started at the same nucleotide, which was previously determined by in vivo S1 analysis. Therefore, it is suggested that accurate transcription of the rRNA gene occurred in this system.

引用

页码：187 / 190

页数：4

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