THE SERUM RESPONSE ELEMENT AND AN AP-1/ATF SEQUENCE IMMEDIATELY DOWNSTREAM COOPERATE IN THE REGULATION OF C-FOS TRANSCRIPTION

Transcription of the c-fos gene is activated in response to a wide variety of extracellular stimuli and several cis-acting transcriptional control elements have been characterized. One of these elements is called the serum response element (SRE) and here we investigate an interaction between this element and an AP-1/ATF-like sequence immediately downstream from the SRE. In growing cells these sequences activate transcription in an additive fashion whereas in quiescent cells they co-operate to repress transcription. This co-operation is disrupted upon separation of the elements which also alters the response of the elements to serum or 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulation of quiescent cells. This separation also results in an increase of transcription in growing cells. A consensus AP-1 DNA-binding site can substitute for the AP-1/ATF-like sequence present in the c-fos promoter to activate transcription in an additive fashion with the SRE in growing cells, and co-operate in repression in quiescent cells. These observations show that any interaction that may be occurring between proteins binding to these elements results in a different pattern of transcriptional control in growing and quiescent cells. Alternatively, different proteins (or modified proteins) may complex with these sequences in the two different states of cell growth.