OPTIMIZATION OF MEDIUM FOR EXTRACELLULAR NUCLEASE FORMATION FROM RHIZOPUS-STOLONIFER

被引：10

作者：

APTE, PS ^{[1
]}

DESHPANDE, MV ^{[1
]}

SHANKAR, V ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1993年 / 9卷 / 02期

关键词：

DNAASE; EXTRACELLULAR NUCLEASE; MEDIUM OPTIMIZATION; RHIZOPUS-STOLONIFER;

D O I：

10.1007/BF00327838

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strain of Rhizopus stolonifer produced a high activity of extracellular DNAase when grown on YPG (yeast extract peptone glucose) medium. The source of peptone had a marked effect on the enzyme yield and only one peptone (i.e. from Sarabhai M. Chemicals Ltd, India) supported enzyme production. Maximum enzyme activity (88 U/ml) was obtained after 4 days' growth under submerged conditions in YPG medium containing 100 muM Mn2+, Co2+ or Mg2+, and glucose as the sole carbon source. The unpurified enzyme was optimally active at pH 7.5 and 45-degrees-C. It had a higher activity with sonicated and heat-denatured DNA than native DNA.

引用

页码：205 / 209

页数：5

共 16 条

[1] Ando T., A nuclease specific for heat-denatured DNA isolated from a product of Aspergillus oryzae, Biochimica et Biophysica Acta, 114, pp. 158-168, (1966)
[2] Curtis P.J., Burdon M.G., Smellie R.M.S., The purification from rat liver of a nuclease hydrolyzing ribonucleic acid and deoxyribonucleic acid, Biochemical Journal, 98, pp. 813-817, (1966)
[3] Dalto M., Pisano M.A., The effect of various peptones on the production of Cephalosporin C by Paecilomyces persicinus P-10, Biotechnology Letters, 13, pp. 637-640, (1991)
[4] Fraser M.J., Alkaline deoxyribonucleases released from Neurospora crassa mycelia: two activities not released by mutants with multiple sensitivities to mutagens, Nucleic Acids Research, 6, pp. 231-246, (1979)
[5] Fujimoto M., Kuninaka A., Yoshino H., Purification of a nuclease from Penicillium citrinum, Agricultural and Biological Chemistry, 38, pp. 777-783, (1974)
[6] Gite S., Reddy G., Shankar V., Active site characterization of S1 nuclease I: Affinity purification and influence of amino group modification, Biochemical Journal, 285, pp. 489-494, (1992)
[7] Linn S., Deoxyribonucleases: Survey and Perspectives, The Enzymes, pp. 121-135, (1981)
[8] Linn S., Lehman I.R., An endonuclease from Neurospora crassa specific for polynucleotides lacking an ordered structure. I. Purification and properties of the enzyme, Journal of Biological Chemistry, 240, pp. 1287-1293, (1965)
[9] Mehra U., Ranjekar P.K., Buffalo (Bos bubalus) genome: Occurrence and characterization of repeated DNA sequences, Indian Journal of Biochemistry and Biophysics, 16, pp. 56-60, (1979)
[10] Mishra N.C., Tatum E.L., Effect of L-sorbose on polysaccharide synthetases of Neurospora crassa, Proceedings of the National Academy of Sciences of the United States of America, 69, pp. 313-317, (1972)

← 1 2 →