CELLULASES AS CHIRAL SELECTORS

被引：39

作者：

ISAKSSON, R

PETTERSSON, C

PETTERSSON, G

JONSSON, S

STALBERG, J

HERMANSSON, J

MARLE, I

机构：

[1] UPPSALA UNIV, CTR BIOMED, DEPT MOLEC BIOL, S-75124 UPPSALA, SWEDEN

[2] CHROMTECH AB, S-12906 HAGERSTEN, SWEDEN

[3] ASTRA PAIN CONTROL AB, PHARMACEUT & ANALYT R&D, S-15185 SODERTALJE, SWEDEN

[4] UNIV UPPSALA, CTR BIOMED, DEPT BIOCHEM, S-75123 UPPSALA, SWEDEN

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 1994年 / 13卷 / 10期

关键词：

D O I：

10.1016/0165-9936(94)85024-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cellulases are powerful chiral selectors especially for the separation of basic drugs. They have found applications both in liquid chromatography and capillary electrophoresis. Cellulases, which are produced in large amounts, are well characterised and thus useful for fundamental studies of the chiral recognition mechanism using, for instance, computer graphics. In this article, some guidelines and procedures are given for the optimisation of enantioselective separations by the mobile phase composition (e.g., pH, charged and uncharged organic modifiers). The influence of solute structure on the enantioselectivity obtained on the cellulase phase is also discussed.

引用

页码：431 / 439

页数：9

共 41 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].