6-KETOCHOLESTANOL ABOLISHES THE EFFECT OF THE MOST POTENT UNCOUPLERS OF OXIDATIVE-PHOSPHORYLATION IN MITOCHONDRIA

被引：57

作者：

STARKOV, AA ^{[1
]}

DEDUKHOVA, VI ^{[1
]}

SKULACHEV, VP ^{[1
]}

机构：

[1] AN BELOZERSKY PHYS CHEM BIOL LAB,DEPT BIOENERGET,MOSCOW 119899,RUSSIA

来源：

FEBS LETTERS | 1994年 / 355卷 / 03期

关键词：

UNCOUPLING; 6-KETOCHOLESTANOL; MITOCHONDRION; CARBOXYATRACTYLATE;

D O I：

10.1016/0014-5793(94)01211-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The effect of a keto-derivative of cholesterol, namely, 6-ketocholestanol (5 alpha-cholestan-3 beta-ol-6-one; kCh) on the uncoupling of oxidation and phosphorylation by various uncouplers was studied in rat heart mitochondria. kCh was found to completely abolish the uncoupling effect (the increase in the respiration rate under the respiratory control conditions and the decrease in the membrane potential) caused of FCCP, CCCP and SF6847 and partially by TTFB at low concentrations of uncouplers. It was without effect on the uncoupling by PCP, DNP and palmitate. Carboxyatractylate, a specific inhibitor of the ADP/ATP-antiporter, was shown to almost completely abolish the uncoupling induced by palmitate and partially by low concentration of TTFB, PCP and DNP. Effects of high concentrations of all these uncouplers as well as of any concentrations of gramicidin proved to be kCh- and carboxyatractilate-insensitive. The data are discussed in terms of the hypothesis on the protein-mediated mechanism of the protonophorous uncoupling.

引用

页码：305 / 308

页数：4

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