NONRADIOACTIVE DNA PROBE FOR DETECTION OF GENE FOR METHICILLIN RESISTANCE IN STAPHYLOCOCCUS-AUREUS

被引：33

作者：

LIGOZZI, M

ROSSOLINI, GM

TONIN, EA

FONTANA, R

机构：

[1] UNIV VERONA, INST MICROBIOL, I-37100 VERONA, ITALY

[2] UNIV PADUA, INST MICROBIOL, I-35100 PADUA, ITALY

[3] UNIV SIENA, INST MICROBIOL, I-53100 SIENA, ITALY

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1991年 / 35卷 / 03期

关键词：

D O I：

10.1128/AAC.35.3.575

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.

引用

页码：575 / 578

页数：4

共 24 条

[1] DETECTION OF METHICILLIN RESISTANCE IN STAPHYLOCOCCI BY USING A DNA PROBE [J].

ARCHER, GL ;

PENNELL, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (09) :1720-1724

[2] INACTIVATION OF BENZYLPENICILLIN AND METHICILLIN BY HOSPITAL STAPHYLOCOCCI [J].

AYLIFFE, GAJ ;

BARBER, M .

BMJ-BRITISH MEDICAL JOURNAL, 1963, (535) :202-+

[3] INTRINSIC RESISTANCE TO BETA-LACTAM ANTIBIOTICS IN STAPHYLOCOCCUS-AUREUS [J].

BROWN, DFJ ;

REYNOLDS, PE .

FEBS LETTERS, 1980, 122 (02) :275-278

[4]

CHAMBERS H F, 1988, Clinical Microbiology Reviews, V1, P173

[5]

FROGGATT JW, 1989, ANTIMICROB AGENTS CH, V31, P1683

[6] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[7] LOW-AFFINITY PENICILLIN-BINDING PROTEIN ASSOCIATED WITH BETA-LACTAM RESISTANCE IN STAPHYLOCOCCUS-AUREUS [J].

HARTMAN, BJ ;

TOMASZ, A .

JOURNAL OF BACTERIOLOGY, 1984, 158 (02) :513-516

[8]

INGLIS B, 1988, J GEN MICROBIOL, V134, P1465

[9]

Kloos W.E., 1985, MANUAL CLIN MICROBIO, P143

[10] SUSCEPTIBILITY OF PENICILLINASE-RESISTANT PENICILLINS AND CEPHALOSPORINS TO PENICILLINASE OF STAPHYLOCOCCUS-AUREUS [J].

LACEY, RW ;

STOKES, A .

JOURNAL OF CLINICAL PATHOLOGY, 1977, 30 (01) :35-39

← 1 2 3 →