CLONING AND SEQUENCING OF 2 ACTIN GENES FROM TAENIA-SOLIUM (CESTODA)

被引:33
作者
CAMPOS, A
BERNARD, P
FAUCONNIER, A
LANDA, A
GOMEZ, E
HERNANDEZ, R
WILLMS, K
LACLETTE, JP
机构
[1] NATL AUTONOMOUS UNIV MEXICO,INST INVEST BIOMED,DEPT IMMUNOL,MEXICO CITY 04510,DF,MEXICO
[2] NATL AUTONOMOUS UNIV MEXICO,INST INVEST BIOMED,DEPT MOLEC BIOL,MEXICO CITY 04510,DF,MEXICO
关键词
cDNA cloning; Cestode actin DNA sequencing; Gene cloning; Taenia solium;
D O I
10.1016/0166-6851(90)90082-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genomic and cDNA actin clones were isolated from Taenia solium gene libraries. The actin genes are interrupted by intervening sequences. Protein coding regions of both genes predict the same amino acid sequence. cDNA sequence data indicate that at least one gene is expressed at the larval stage. Results from Northern and Western blots showed that T. solium expresses an actin transcript of about 1 400 bases and a protein of 45 000 Da. © 1990.
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页码:87 / 93
页数:7
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