GLYCEROL PERMEABILITY OF HUMAN SPERMATOZOA AND ITS ACTIVATION-ENERGY

被引:45
作者
GAO, DY
MAZUR, P
KLEINHANS, FW
WATSON, PF
NOILES, EE
CRITSER, JK
机构
[1] INDIANA UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS,INDIANAPOLIS,IN 46202
[2] INDIANA UNIV,SCH MED,DEPT OBSTET & GYNECOL,INDIANAPOLIS,IN 46202
[3] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37831
[4] INDIANA UNIV PURDUE UNIV,DEPT PHYS,INDIANAPOLIS,IN 46205
[5] UNIV LONDON ROYAL VET COLL,DEPT VET BASIC SCI,LONDON NW1 0TU,ENGLAND
关键词
D O I
10.1016/0011-2240(92)90068-D
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Glycerol has commonly been employed as a cryoprotectant in cryopreservation of human spermatozoa. However, the addition of glycerol into the sperm before freezing and the removal of glycerol from the sperm after freezing and thawing result in anisotonic environments to the cells, which can cause cell injury. To define optimal procedures for the addition/removal of glycerol and to minimize the cell injury, one needs to know the kinetics of glycerol permeation across the sperm plasma membrane at different temperatures. For this, one has to determine the permeability coefficient of glycerol (Pg) and its activation energy (Ea). Values of Pg at different temperatures and at different glycerol concentrations were determined by measuring the time required for 50% spermolysis in hyperosmotic glycerol solutions which were hypotonic with respect to electrolytes. Value of the Ea was determined assuming an Arrhenius type temperature dependence of Pg. A dual fluorescent staining technique (propidium iodide and 6-carboxyfluoroscein diacetate) and flow cytometry were used to measure the spermolysis. The values of Pg in 0.5, 1.0, 1.5, and 2.0 M glycerol at 22 °C are 1.62, 1.88, 1.68, and 1.54 × 10-3 cm/min, respectively. The values of Pg in 1 M glycerol at 0, 8, 22, and 30 °C are 0.33, 0.54, 1.88, and 2.60 × 10-3 cm/min, respectively. The value of Ea is 11.76 kcal/mol. © 1992.
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页码:657 / 667
页数:11
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