TRANSCRIPTION OF HUMAN RESPIRATORY SYNCYTIAL VIRUS GENOME RNA INVITRO - REQUIREMENT OF CELLULAR FACTOR(S)

被引：78

作者：

BARIK, S

机构：

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 11期

关键词：

D O I：

10.1128/JVI.66.11.6813-6818.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Extracts made from human respiratory syncytial virus (RSV)-infected Hep-2 cells synthesized mRNAs encoded by all known viral genes. In contrast, RSV ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected Hep-2 cells, demonstrating that a cellular factor(s) has a role in RSV gene expression. Quantitation of the individual gene mRNAs transcribed in vitro revealed polarity of transcription of the genome.

引用

页码：6813 / 6818

页数：6

共 35 条

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