IODIXANOL - A NONIONIC ISOSMOTIC CENTRIFUGATION MEDIUM FOR THE FORMATION OF SELF-GENERATED GRADIENTS

被引：128

作者：

FORD, T ^{[1
]}

GRAHAM, J ^{[1
]}

RICKWOOD, D ^{[1
]}

机构：

[1] UNIV ESSEX,DEPT BIOL,COLCHESTER CO4 3SQ,ESSEX,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 220卷 / 02期

关键词：

D O I：

10.1006/abio.1994.1350

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The physical and biological properties of Iodixanol, a new nonionic density gradient medium, are described in this paper. It is effectively a dimer of Nycodenz and it exhibits two significant advantages over previous iodinated density gradient media-its aqueous solutions are iso-osmotic up to a density of 1.32 g/ml and it is capable of forming self-generating gradients in 1 to 3 h. It has a very low toxicity toward biological material and enzyme assays can be carried out in its presence. (C) 1994 Academic Press, Inc.

引用

页码：360 / 366

页数：7

共 29 条

[1] ISOLATION OF HIGHLY PURIFIED LYSOSOMES FROM RAT-LIVER - IDENTIFICATION OF ELECTRON CARRIER COMPONENTS ON LYSOSOMAL MEMBRANES [J].