EPSTEIN-BARR-VIRUS (EBV) REPLICATIVE GENE-EXPRESSION IN TUMOR-CELLS OF AIDS-RELATED NON-HODGKINS-LYMPHOMA IN RELATION TO CD4 CELL NUMBER AND ANTIBODY-TITERS TO EBV

被引:13
作者
BROUSSET, P
DROUET, E
SCHLAIFER, D
ICART, J
PAYEN, C
MEGGETTO, F
MARCHOU, B
MASSIP, P
DELSOL, G
机构
[1] CHU PURPAN,CIGH,CNRS,TOULOUSE,FRANCE
[2] INST PASTEUR,DEPT VIROL,LYON,FRANCE
[3] CHU PURPAN,DEPT HAEMATOL,TOULOUSE,FRANCE
[4] CHU RANGUEIL,DEPT VIROL,RANGUEIL,FRANCE
[5] CHU PURPAN,DEPT INFECT DIS,TOULOUSE,FRANCE
关键词
AIDS; LYMPHOMA; EPSTEIN-BARR VIRUS; REPLICATIVE GENES; SEROLOGY; ZEBRA PROTEIN; CD4 CELL COUNT;
D O I
10.1097/00002030-199405000-00003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Objective: To determine whether activation of Epstein-Barr virus (EBV) replication in tumour cells of AIDS-related non-Hodgkin's lymphoma (ARNHL) is correlated with CD4+ cell counts and influences antibody response to EBV [anti-Z Epstein-Barr replicative activator (ZEBRA), anti-early antigen (EA), anti-viral capsid antigen (VCA)]. Design: Retrospective study based on immunohistochemistry and in situ hybridization to detect EBV replicative gene products in tissue samples from patients affected by ARNHL and correlation with CD4+ cell counts and results of EBV serology (including anti-ZEBRA activity) in sera from the same patients. Methods: Seventeen out of 22 cases of ARNHL were selected for the presence of EBV [Epstein-Barr early region (EBER) RNA-positive]. Immunohistochemistry was performed with anti-ZEBRA, anti-EA-restricted, anti-VCA antibodies and in situ hybridization with BHLF1/Notl oligoprobes on tumour samples. Results were statistically correlated with those of CD4+ cell counts (17 out of 17) and with anti-EBV antibody titres (13 out of 17) assessed using standard immunofluorescence method and enzyme-linked immunosorbent assay procedure using recombinant ZEBRA protein and synthetic peptides as antigens. Results: BZLF1 (ZEBRA) or early gene products (EA-R and EA-D/BHLF1/Notl) were detected in a small proportion (<0.01-5%) of tumour cells in eight of these 17 cases by immunohistochemistry and in situ hybridization. Demonstration of replicative gene expression did not correlate with either low CD4+ cell counts (P>0.05) or anti-EBV antibody titres (P>0.05). Anti-ZEBRA activity was not significantly increased in patients affected with ARNHL, the cells of which expressed replicative gene products (P>0.05). Conclusion: The degree of immunodeficiency does not clearly enhance replicative gene expression in tumour cells of ARNHL. EBV serology, including anti-ZEBRA activity, is not a reliable tool for predicting the occurrence of such proliferations.
引用
收藏
页码:583 / 590
页数:8
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