ISOLATION AND CHARACTERIZATION OF A PROTEINASE FROM WHITE GOURD

被引：29

作者：

KANEDA, M ^{[1
]}

TOMINAGA, N ^{[1
]}

机构：

[1] KAGOSHIMA UNIV, FAC SCI, DEPT CHEM, KAGOSHIMA, KAGOSHIMA 890, JAPAN

来源：

PHYTOCHEMISTRY | 1977年 / 16卷 / 03期

关键词：

D O I：

10.1016/0031-9422(77)80061-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A proteinase from the sarcocarp of Benincasa cerifera was purified. Its MW was estimated by 2 different methods to be about 50,000. The maximum activity was found in the alkaline pH region against casein as a substrate. The enzyme was strongly inhibited by di-isopropyl fluorophosphate and was not inhibited by EDTA and p-chloromercuribenzoic acid.

引用

页码：345 / 346

页数：2

共 7 条

[1]

Deb-Sarma G. D., 1942, ANN BIOCHEM AND EXP MED [CALCUTTA], V2, P197

[2] ISOLATION AND CHARACTERIZATION OF A PROTEINASE FROM SARCOCARP OF MELON FRUIT [J].

KANEDA, M ;

TOMINAGA, N .

JOURNAL OF BIOCHEMISTRY, 1975, 78 (06) :1287-1296

[3] CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR .2. GENERAL PROPERTIES [J].

KUNITZ, M .

JOURNAL OF GENERAL PHYSIOLOGY, 1947, 30 (04) :291-310

[4]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[5] MOLECULAR WEIGHT ESTIMATION OF POLYPEPTIDE CHAINS BY ELECTROPHORESIS IN SDS-POLYACRYLAMIDE GELS [J].

SHAPIRO, AL ;

VINUELA, E ;

MAIZEL, JV .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 28 (05) :815-&

[6]

Walsh K.A., 1970, METHODS ENZYMOLOGY P, VXIX., P31, DOI DOI 10.1016/0076-6879(70)19005-7

[7]

WEBER K, 1969, J BIOL CHEM, V244, P4406

← 1 →