HIGH-LEVEL SOLUBLE EXPRESSION OF ISOPENICILLIN-N SYNTHASE ISOZYMES IN ESCHERICHIA-COLI

被引：21

作者：

BALDWIN, JE ^{[1
]}

BLACKBURN, JM ^{[1
]}

SUTHERLAND, JD ^{[1
]}

WRIGHT, MC ^{[1
]}

机构：

[1] OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

TETRAHEDRON | 1991年 / 47卷 / 31期

关键词：

D O I：

10.1016/S0040-4020(01)86491-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The Isopenicillin N Synthase genes from Cephalosporium acremonium, Penicillium chrysogenum and Aspergillus nidulans have been expressed at high levels in Escherichia coli under control of the trc or lac promoters. The enzymes are produced in soluble form in contrast to previously reported expression in insoluble form under control of the lambda-P(L) promoter.

引用

页码：5991 / 6002

页数：12

共 30 条

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