STEADY-STATE POLARIZED-LIGHT SPECTROSCOPY OF ISOLATED PHOTOSYSTEM-I COMPLEXES

被引:68
作者
VANDERLEE, J
BALD, D
KWA, SLS
VANGRONDELLE, R
ROGNER, M
DEKKER, JP
机构
[1] FREE UNIV AMSTERDAM,DEPT PHYS & ASTRON,DE BOELELAAN 1081,1081 HV AMSTERDAM,NETHERLANDS
[2] UNIV MUNSTER,INST BOT,W-4400 MUNSTER,GERMANY
关键词
PHOTOSYSTEM-I; SPECTROSCOPY; FLUORESCENCE; SYNECHOCYSTIS;
D O I
10.1007/BF00016562
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Monomeric and trimeric Photosystem I core complexes from the cyanobacterium Synechocystis PCC 6803 and LHC-I containing Photosystem I (PS I-200) complexes from spinach have been characterized by steady-state, polarized light spectroscopy at 77 K. The absorption spectra of the monomeric and trimeric core complexes from Synechocystis were remarkably similar, except for the amplitude of a spectral component at long wavelength, which was about twice as large in the trimeric complexes. This spectral component did not contribute significantly to the CD-spectrum. The (77 K) steady-state emission spectra showed prominent peaks at 724 nm (for the Synechocystis core complexes) and at 735 nm (for PS I-200). A comparison of the excitation spectra of the main emission band and the absorption spectra suggested that a significant part of the excitations do not pass the red pigments before being trapped by P-700. Polarized fluorescence excitation spectra of the monomeric and trimeric core complexes revealed a remarkably high anisotropy (approximately 0.3) above 705 nm. This suggested one or more of the following possibilities: 1) there is one red-most pigment to which all excitations are directed, 2) there are more red-most pigments but with (almost) parallel orientations, 3) there are more red-most pigments, but they are not connected by energy transfer. The high anisotropy above 705 nm of the trimeric complexes indicated that the long-wavelength pigments on different monomers are not connected by energy transfer. In contrary to the Synechocystis core complexes, the anisotropy spectrum of the LHC I containing complexes from spinach was not constant in the region of the long-wavelength pigments, and decreased significantly below 720 nm, the wavelength where the long-wavelength pigments on the core complexes start to absorb. These results suggested that in spinach the long-wavelength pigments on core and LHC-I are connected by energy transfer and have a non-parallel average Q(y(0-0)) transitions.
引用
收藏
页码:311 / 321
页数:11
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