SEPARATION OF CLOSELY-RELATED INTRINSIC MEMBRANE POLYPEPTIDES OF THE PHOTOSYSTEM-II LIGHT-HARVESTING COMPLEX (LHC-II) BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A POLY(STYRENE-DIVINYLBENZENE) COLUMN

被引：8

作者：

DAMM, I ^{[1
]}

GREEN, BR ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1Z4,BC,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 664卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/0021-9673(94)80625-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The three closely related intrinsic membrane polypeptides of the photosystem II light-harvesting complex (LHC II) were successfully resolved on a PRP-1 poly(styrene-divinylbenzene) column using a three-stage linear water-acetonitrile gradient containing 0.1 % trifluoroacetic acid. The hydrophobic proteins of photosystem I (PS I-200) and photosystem II core particles were also separated by this method, showing that membrane proteins of different sizes and hydrophobicities can be resolved in this system.

引用

页码：33 / 38

页数：6

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