MONOCLONAL-ANTIBODIES TO HIGH-INCIDENCE KELL EPITOPES - CHARACTERIZATION AND APPLICATION IN AUTOMATED SCREENING OF DONOR SAMPLES

Monoclonal antibodies, LM313/706 and LM357/828, recognize high-frequency epitopes which are absent on red cells of Ko phenotype. Both antibodies have proved suitable for automated screening of blood donor samples, with 5 ml of each culture supernatant sufficient to screen 3,000 donor samples. In a screen of 45,545 samples, LM313/706 revealed 11 samples (1:3686) of Kp(a+b-) phenotype. Thirty-seven samples of K+k- phenotype were identified in 15,235 samples screened with LM357/828 antibody (0.24%). The epitope recognized by LM313/706 was inactivated by 2 mM DTT suggesting a possible association with the Js(a)/Js(b) antigen structure. The expression of the k-like epitope detected by LM357/828 antibody was found to be influenced by pH change.