CAULIFLOWER MOSAIC-VIRUS P35S PROMOTER ACTIVITY IN ESCHERICHIA-COLI

被引：58

作者：

ASSAAD, FF ^{[1
]}

SIGNER, ER ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 223卷 / 03期

关键词：

CaMV P35S promoter; Escherichia coli; Kanamycin resistance; Transcription initiation;

D O I：

10.1007/BF00264462

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present evidence that the cauliflower mosaic virus promoter P35S can direct expression of the bacterial neomycin phosphotransferase II (NPTll) gene in . Transcription is initiated at several sites, the major one being located approximately 315 bases upstream of the plant start site. The nucleotide sequence directly preceding this start site is strongly homologous to the prokaryotic promoter consensus sequence. Thus constructs designed for introduction into plants can be expressed in E. coli. © 1990 Springer-Verlag.

引用

页码：517 / 520

页数：4

共 15 条

[1] Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210
[2] REGULATED GENES IN TRANSGENIC PLANTS
BENFEY, PN
CHUA, NH
[J]. SCIENCE, 1989, 244 (4901) : 174 - 181
[3] THE CAMV S-35 ENHANCER CONTAINS AT LEAST 2 DOMAINS WHICH CAN CONFER DIFFERENT DEVELOPMENTAL AND TISSUE-SPECIFIC EXPRESSION PATTERNS
BENFEY, PN
REN, L
CHUA, NH
[J]. EMBO JOURNAL, 1989, 8 (08) : 2195 - 2202
[4] MAPPING OF THE COAT PROTEIN GENE OF CAULIFLOWER MOSAIC-VIRUS BY ITS EXPRESSION IN A PROKARYOTIC SYSTEM
DAUBERT, S
RICHINS, R
SHEPHERD, RJ
GARDNER, RC
[J]. VIROLOGY, 1982, 122 (02) : 444 - 449
[5] EXPRESSION OF GENES TRANSFERRED INTO MONOCOT AND DICOT PLANT-CELLS BY ELECTROPORATION
FROMM, M
TAYLOR, LP
WALBOT, V
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (17) : 5824 - 5828
[6] THE COMPLETE NUCLEOTIDE-SEQUENCE OF AN INFECTIOUS CLONE OF CAULIFLOWER MOSAIC-VIRUS BY M13MP7 SHOTGUN SEQUENCING
GARDNER, RC
HOWARTH, AJ
HAHN, P
BROWNLUEDI, M
SHEPHERD, RJ
MESSING, J
[J]. NUCLEIC ACIDS RESEARCH, 1981, 9 (12) : 2871 - 2888
[7] TRANSCRIPTION OF CAULIFLOWER MOSAIC-VIRUS DNA - DETECTION OF PROMOTER SEQUENCES, AND CHARACTERIZATION OF TRANSCRIPTS
GUILLEY, H
DUDLEY, RK
JONARD, G
BALAZS, E
RICHARDS, KE
[J]. CELL, 1982, 30 (03) : 763 - 773
[8] EXPRESSION OF CHIMAERIC GENES TRANSFERRED INTO PLANT-CELLS USING A TI-PLASMID-DERIVED VECTOR
HERRERAESTRELLA, L
DEPICKER, A
VANMONTAGU, M
SCHELL, J
[J]. NATURE, 1983, 303 (5914) : 209 - 213
[9] LOCALIZED TRANSIENT EXPRESSION OF GUS IN LEAF-DISKS FOLLOWING COCULTIVATION WITH AGROBACTERIUM
JANSSEN, BJ
GARDNER, RC
[J]. PLANT MOLECULAR BIOLOGY, 1990, 14 (01) : 61 - 72
[10] ISOLATION AND MAPPING OF SMALL CAULIFLOWER MOSAIC-VIRUS DNA FRAGMENTS ACTIVE AS PROMOTERS IN ESCHERICHIA-COLI
MCKNIGHT, TD
MEAGHER, RB
[J]. JOURNAL OF VIROLOGY, 1981, 37 (02) : 673 - 682

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