SPONTANEOUS ARRHYTHMIAS IN VARIOUS MODELS OF CARDIAC-HYPERTROPHY AND SENESCENCE OF RATS - A HOLTER MONITORING STUDY

Objective: The aim was to define experimental models of spontaneous arrhythmias in various models of cardiac hypertrophy in rats. Methods: Cardiac hypertrophy was induced by several methods and 24 h Holter monitoring was recorded in conscious rats to quantify spontaneous arrhythmias in hypertrophied hearts. Male Wistar rats were studied. A group of young controls 1-2 months old (n=16) was compared to four groups of animals with cardiac hypertrophy: (1) thyrotoxic rats which received a daily intraperitoneal injection of L-thyroxine for 7 d (n=6); (2) rats subjected to abdominal suprarenal aortic stenosis (n=11); (3) senescent rats 22-24 month old (n=6); and (4) S-DOCA-salt (senescent animals rendered hypertensive by uninephrectomy and DoCA-salt treatment, n=8). Results: (1) Thyroxine resulted in 20% cardiac hypertrophy, with normal arterial tension, sinus tachycardia, a shorter P wave length and PR interval, and frequent (5/6) atrioventricular block. No premature beats were seen. (2) In aortic stenosis, atria and left ventricle were hypertrophied by 53% and systolic carotid pressure increased by 63%. The incidence of supraventricular premature beats was increased [frequency=0.70 (SEM 0.3) per 24 h in control v 99(61) in aortic stenosis, p<0.05]. Ventricular premature beats remained as rare as in control. (3) In senescent and S-DOCA-salt rats all types of spontaneous arrhythmias, but specially supraventricular arrhythmias and atrioventricular block, were frequent. Cardiac hypertrophy produced by DOCA-salt treatment in senescent rats had no effect on the incidence and nature of arrhythmias, but resulted in an increased QTc interval. Conclusions: Senescent rats and rats with aortic stenosis represent valid models of spontaneous arrhythmias occurring in the absence of ischaemia or toxic insult. Spontaneous arrhythmias in rats are mainly of supraventricular origin. Hyperthyroidism in rats is a model of atrioventricular block probably related to tachycardia. Holter monitoring in rats may have several potential pathophysiological and pharmacological applications.