IMMUNOLOGICAL CHARACTERIZATION OF NEUTRAL CHOLESTERYL ESTER HYDROLASE FROM RAT-LIVER CYTOSOL

被引：14

作者：

GHOSH, S ^{[1
]}

GROGAN, WM ^{[1
]}

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOCHEM & MOLEC BIOPHYS,BOX 614,MCV STN,RICHMOND,VA 23298

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1992年 / 70卷 / 09期

关键词：

CHOLESTERYL ESTERASE; POLYCLONAL ANTIBODIES; RAT LIVER; SUBCELLULAR DISTRIBUTION;

D O I：

10.1139/o92-121

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit polyclonal antibodies were raised against rat liver bile salt-independent neutral cholesteryl ester hydrolase (CEH) and used for subcellular localization and immunological comparison with isoforms from other tissues. Antibodies exhibited a high degree of specificity for the liver CEH through all stages of purification and neutralized 70-80% of the activity of liver cytosolic CEH. They exhibited various levels of cross-reactivity with cytosolic proteins from other tissues, but reacted weakly with pancreatic and intestinal proteins and did not inhibit pancreatic CEH. Cytosol contained 78% of total cellular CEH activity and 75% of CEH immunoreactive protein. Washed microsomes contained 3% of CEH activity and 5% of CEH protein.

引用

页码：800 / 803

页数：4

共 17 条

[1] HORMONAL DEPENDENCE OF CHOLESTEROL ESTER HYDROLASE IN CORPUS-LUTEUM AND ADRENAL [J].