GUANINE-NUCLEOTIDE EXCHANGE FACTOR FOR EUKARYOTIC INITIATION FACTOR-II - CLONING OF CDNA FOR THE DELTA-SUBUNIT OF RABBIT TRANSLATION INITIATION FACTOR-2B

被引：15

作者：

PRICE, NT

FRANCIA, G

HALL, L

PROUD, CG

机构：

[1] Department of Biochemistry, University of Bristol, Bristol

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1217卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

PROTEIN SYNTHESIS; INITIATION FACTOR-2B; EUKARYOTE; GUANINE NUCLEOTIDE EXCHANGE FACTOR;

D O I：

10.1016/0167-4781(94)90037-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide sequence data for rabbit eIF-2B delta were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2B delta transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5' sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2B delta, the GCD2 gene product.

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收藏

页码：207 / 210

页数：4

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