ANEUPLOIDY INDUCED BY CHLORAL HYDRATE DETECTED IN HUMAN-LYMPHOCYTES WITH THE Y97 PROBE

被引：22

作者：

VAGNARELLI, P

DESARIO, A

DECARLI, L

机构：

[1] Dipartimento di Genetica e Microbiologia 'A. Buzzati-Traverso', University of Pavia, Pavia

来源：

MUTAGENESIS | 1990年 / 5卷 / 06期

关键词：

D O I：

10.1093/mutage/5.6.591

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chloral hydrate (CH) has been assayed for its ability to induce chromosome number variation in human lymphocytes in culture. Aneuploidy induction has been detected by means of in situ hybridization on interphase nuclei with a chromosome Y-specific DNA probe. A dose-dependent increase in the number of hyperdiploid nuclei was found at CH concentrations ranging from 250 to 750 μg/ml. These results represent a further contribution to the validation of the method which gave a positive response with drugs characterized by different mechanisms of action and therefore may be of general use in detecting aneuploidy in mammalian cells. © 1990 Oxford University Press.

引用

收藏

页码：591 / 592

页数：2

相关论文

共 10 条

[1] MUTAGENICITY OF TRICHLOROETHYLENE, TRICHLOROETHANOL AND CHLORAL HYDRATE IN ASPERGILLUS-NIDULANS [J].

CREBELLI, R ;

CONTI, G ;

CONTI, L ;

CARERE, A .

MUTATION RESEARCH, 1985, 155 (03) :105-111

[2] ANEUPLOIDY ASSAY ON DIETHYLSTILBESTROL BY MEANS OF INSITU HYBRIDIZATION OF RADIOACTIVE AND BIOTINYLATED DNA PROBES ON INTERPHASE NUCLEI [J].

DESARIO, A ;

VAGNARELLI, P ;

DECARLI, L .

MUTATION RESEARCH, 1990, 243 (02) :127-131

[3] CHEMICALLY-INDUCED ANEUPLOIDY IN MAMMALIAN-CELLS IN CULTURE [J].

GALLOWAY, SM ;

IVETT, JL .

MUTATION RESEARCH, 1986, 167 (1-2) :89-105

[4]

LIANG JC, 1985, ANEUPLOIDY ETIOLOGY, P491

[5] DNA PROBE LOCALIZATION AT 18P113 BAND BY INSITU HYBRIDIZATION AND IDENTIFICATION OF A SMALL SUPERNUMERARY CHROMOSOME [J].

MATTEI, MG ;

PHILIP, N ;

PASSAGE, E ;

MOISAN, JP ;

MANDEL, JL ;

MATTEI, JF .

HUMAN GENETICS, 1985, 69 (03) :268-271

[6]

Raimondi E, 1987, Cytotechnology, V1, P13, DOI 10.1007/BF00351116

[7] ANEUPLOIDY ASSAYS ON INTERPHASE NUCLEI BY MEANS OF INSITU HYBRIDIZATION WITH DNA PROBES [J].

RAIMONDI, E ;

SCARIOLO, S ;

DESARIO, A ;

DECARLI, L .

MUTAGENESIS, 1989, 4 (03) :165-169

[8] NONDISJUNCTION INDUCED IN MOUSE SPERMATOGENESIS BY CHLORAL HYDRATE, A METABOLITE OF TRICHLOROETHYLENE [J].

RUSSO, A ;

PACCHIEROTTI, F ;

METALLI, P .

ENVIRONMENTAL MUTAGENESIS, 1984, 6 (05) :695-703

[9] CHLORAL HYDRATE, METHYLMERCURY HYDROXIDE AND ETHIDIUM-BROMIDE AFFECT CHROMOSOMAL SEGREGATION DURING MEIOSIS OF SACCHAROMYCES-CEREVISIAE [J].

SORA, S ;

CARBONE, MLA .

MUTATION RESEARCH, 1987, 190 (01) :13-17

[10] ISOLATION AND CHARACTERIZATION OF AN ALPHOID CENTROMERIC REPEAT FAMILY FROM THE HUMAN Y-CHROMOSOME [J].

WOLFE, J ;

DARLING, SM ;

ERICKSON, RP ;

CRAIG, IW ;

BUCKLE, VJ ;

RIGBY, PWJ ;

WILLARD, HF ;

GOODFELLOW, PN .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 182 (04) :477-485