PURIFICATION OF HUMAN-LEUKOCYTE DNA - PROTEINASE-K IS NOT NECESSARY

被引：19

作者：

DOUGLAS, AM

GEORGALIS, AM

BENTON, LR

CANAVAN, KL

ATCHISON, BA

机构：

[1] Victorian Institute of Forensic Pathology, South Melbourne, Vic. 3205

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 201卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90352-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis of the cells in SDS followed by extraction with 6 m NaCl. The method described overcomes the requirement for lengthy incubations in the presence of expensive proteinase K and subsequent extraction with toxic chemicals. © 1992.

引用

页码：362 / 365

页数：4

共 6 条

[1]

BUDOWLE B, 1990, Applied and Theoretical Electrophoresis, V1, P181

[2]

KANTER E, 1986, J FORENSIC SCI, V31, P403

[3] RAPID AND SENSITIVE COLORIMETRIC METHOD FOR VISUALIZING BIOTIN-LABELED DNA PROBES HYBRIDIZED TO DNA OR RNA IMMOBILIZED ON NITROCELLULOSE - BIO-BLOTS [J].