MOLECULAR-CLONING AND GENETIC-CHARACTERIZATION OF THE RFB REGION FROM YERSINIA-PSEUDOTUBERCULOSIS SEROGROUP-IIA, WHICH DETERMINES THE FORMATION OF THE 3,6-DIDEOXYHEXOSE ABEQUOSE

被引：22

作者：

KESSLER, AC ^{[1
]}

BROWN, PK ^{[1
]}

ROMANA, LK ^{[1
]}

REEVES, PR ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT MICROBIOL,SYDNEY,NSW 2006,AUSTRALIA

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1991年 / 137卷

关键词：

D O I：

10.1099/00221287-137-12-2689

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The rfb region of Yersinia pseudotuberculosis serogroup IIA has been cloned and expression of O antigen in Escherichia coli K12 was demonstrated. Transposon mutagenesis analysis confined the DNA region required for O antigen expression to a 19.3 kb fragment, and the O antigen expressed was visualized by SDS-PAGE and silver staining. Southern hybridization analysis demonstrated significant levels of similarity between the Yersinia rfb region and the 3,6-dideoxyhexose pathway genes rfbF and rfbG, previously isolated from Salmonella enterica LT2, but no similarity to the abequose synthase gene rfbJ of the same strain of the paratose synthase gene rfbS isolated from S. enterica Ty2. The evolutionary relationship between the abequose biosynthetic genes of the two species of Salmonella and Yersinia is discussed.

引用

页码：2689 / 2695

页数：7

共 28 条

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