GENETIC-VARIATION OF MICROSATELLITE MARKERS D1S117, D6S89, D11S35, APOC2, AND D21S168 IN THE SPANISH POPULATION

被引:13
作者
FUENTES, JJ
BANCHS, I
VOLPINI, V
ESTIVILL, X
机构
[1] HOSP DURAN & REYNALS,IRO,CTR MED GENET,DEPT MOLEC GENET,C CASTELLDEFELS,KM 2 7,E-08907 BARCELONA,SPAIN
[2] HOSP CLIN BARCELONA,GENET SERV,E-08036 BARCELONA,SPAIN
关键词
MICROSATELLITES; VNTR; PATERNITY TESTING; POLYMORPHISMS;
D O I
10.1007/BF01370384
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
We have used PCR amplification to analyse the allele frequency, distribution and heterozygosity of 5 microsatellite markers (D1S117, D6S89, D11S35, APOC2, and D21S168), in a sample of 100 unrelated Spanish individuals. The loci tested exhibit wide allelic variability having 7-17 alleles, PIC (polymorphic information content) between 0.79 and 0.86, and heterozygosity between 0.81 and 0.86. D1S117 and D21S168 have unimodal distribution, APOC2 has 4 common alleles which account for 71% of the total variation, D11S35 has a bimodal distribution and D6S89 is trimodal. The allelic distribution observed for each locus is in agreement with slippage and mispairing as the main mechanisms involved in the evolution of microsatellite alleles. Multiplex amplification of loci D6S89 and APOC2 was possible due to their non-overlapping allele sizes. The rapidity with which microsatellites can be analysed, and the accurate determination of alleles, make these markers very powerful tools for genetic typing. The information obtained for loci D1S117, D6S89, D11S35, APOC2, and D21S168, provides a basis for their use for DNA typing and paternity analysis in the Spanish population.
引用
收藏
页码:271 / 277
页数:7
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