ANTIBODY-ANTIGEN BINDING CONSTANTS DETERMINED IN SOLUTION-PHASE WITH THE THRESHOLD MEMBRANE-CAPTURE SYSTEM - BINDING CONSTANTS FOR ANTIFLUORESCEIN, ANTI-SAXITOXIN, AND ANTI-RICIN ANTIBODIES

Affinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 × 10-7 to ∼3 × 10-12 M. Dissociation constants for fluorescein and saxitoxin were compared with values determined by independent means. This technique was found to be quick, simple, reproducible, and accurate. © 1994 Academic Press. All rights reserved.